Pawluk, R.J., T.M. Uren Webster, J. Cable, C. Garcia de Leaniz & S. Consuegra. (2018). Immune-Related Transcriptional Responses to Parasitic Infection in a Naturally Inbred Fish: Roles of Genotype and Individual Variation. Genome Biology and Evolution. 10(1):319-327.

485922

10.1093/gbe/evx274 [view]

Pawluk, R.J., T.M. Uren Webster, J. Cable, C. Garcia de Leaniz & S. Consuegra

2018

10(1):319-327.

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Parasites are strong drivers of evolutionary change and the genetic variation of both host and parasite populations can coevolve as a function of parasite virulence and host resistance. The role of transcriptome variation in speci?c interactions between host and parasite genotypes has been less studied and can be confounded by differences in genetic variation. We employed two naturally inbred lines of a self-fertilizing ?sh to estimate the role of host genotype in the transcriptome response to parasite infection using RNA-seq. In addition, we targeted several differentially expressed immune-related genes to further investigate the relative role of individual variation in the immune response using RT-qPCR, taking advantage of the genomic uniformity of the self-fertilizing lines. We found signi?cant differences in gene expression between lines in response to infection both in the transcriptome and in individual gene RT-qPCR analyses. Individual RT-qPCR analyses of gene expression identi?ed signi?cant variance differences between lines for six genes but only for three genes between infected and control ?sh. Our results indicate that although the genetic background plays an important role in the transcriptome response to parasites, it cannot fully explain individual differences within genetically homogeneous lines, which can be important for determining the response to parasites. Key words: Kryptolebias marmoratus, transcriptome, Argulus foliaceus, immune response, inbreeding.

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