Initiation and development of cichlid and zebrafish first-generation teeth: an in vitro study
Huysseune, A.; Sire, J.-Y.; Van der Heyden, C. (1998). Initiation and development of cichlid and zebrafish first-generation teeth: an in vitro study. Biol. Jb. Dodonaea 65: 156
Beeckman, T.; Caemelbeke, K. (Ed.) (1998). Populations: Natural and manipulated, symposium organized by the Royal Society of Natural Sciences Dodonaea, University of Gent, 29 October 1997. Biologisch Jaarboek (Dodonaea), 65. Koninklijk Natuurwetenschappelijk Genootschap Dodonaea: Gent. 257 pp., more
We have used a recently developed organ culture method based on a serum-free culture medium, appropriate for teleost skeletal and dental tissues (KOUMANS & SIRE, 1996), to study the initiation and development of first-generation teeth in early post-embryonic stages of two teleostean fishes, the cichlid Hemichromis bimaculatus (Cichlidae) and the zebrafish Danio rerio (Cyprinidae). Entire heads, explanted for 3 to 7 days reveal (1) the in vitro formation of tooth germs from a microscopically undifferentiated epithelium in the cichlid, but not in the zebrafish, (2) ongoing morphogenesis and cytodifferentiation of initiated tooth germs including the deposition of dentine, and (3) attachment of the tooth through attachment bone deposited in vitro. The pattern observed in early postembryonic stages of the cichlid can be mimicked in organ cultures of the head, but not of isolated jaws. The results point to the possibility of the involvement of neurotrophic factors in tooth initiation in these teleost models.
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