Marine biological valuation provides a comprehensive concept for assessing the intrinsic value of subzones within a study area. This paper gives an update on the concept of marine biological valuation as described by Derous et al. (2007). This concept was based on a literature review of existing ecological valuation criteria and the consensus reached by a discussion group of experts during an international workshop in December 2004. The concept was discussed during an ENCORA-MARBEF workshop in December 2006, which resulted in the fine-tuning of the concept of marine biological valuation, especially with respect to its applicability to marine areas.