De invloed van elektrolyten op de functionele en structurele eigenschappen van haemoglobine van Arenicola marina L.
Everaarts, J.M. (1971). De invloed van elektrolyten op de functionele en structurele eigenschappen van haemoglobine van Arenicola marina L. NIOZ-rapport, 1971(3). Nederlands Instituut voor Onderzoek der Zee: Texel. 46, 14 pp.
Part of: NIOZ-rapport. Netherlands Institute for Sea Research (NIOZ): Den Burg. ISSN 0923-3210
The effects of electrolytes on the oxygen binding characteristics of Arenicola marina haemoglobine is studied.The oxygen affinity of the haemoglobin increases with increasing electrolyte concentration. Increase in electrolyte concentration simultaneously cause an increase in the apparent haem-haem interaction. Again the effect of monovalent cations is smaller than that of divalent ones. The effects of electrolytes on Arenicola haemoglobine show radical differences than on the human pigment.The Bohr effect is measured at different electrolyte concentrations in order to apply pH corrections to the salt effect data.In order to interpret the above data physiologically the electrolyte regulation is studied. The internal concentrations are determined as a function of environmental salinities. Arenicola marina shows slight electrolyte regulation. The blood electrolyte concentration is lower than in the surrounding water, this difference increases with increasing salinity.The results are discussed comparatively, with special reference to salt effects of human Hb for which some qualitative and quantitative data are available.
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