Marine Biodiversity and Ecosystem Functioning
EU Network of Excellence

 
Main Menu

· Home
· Contacts
· Data Systems
· Documents
· FAQ
· Links
· MarBEF Open Archive
· Network Description
· Outreach
· Photo Gallery
· Quality Assurance
· Register of Resources
· Research Projects
· Rules and Guidelines
· Training
· Wiki
· Worldconference

 

Register of Resources (RoR)

 People  |  Datasets  |  Literature  |  Institutes  |  Projects 

[ report an error in this record ]basket (0): add | show Print this page

A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration
Bijnens, K.; Jaenen, V.; Wouters, A.; Leynen, N.; Pirotte, N.; Artois, T.; Smeets, K. (2021). A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration. Biomolecules 11(5): 714. https://dx.doi.org/10.3390/biom11050714
In: Biomolecules. MDPI AG: Basel. e-ISSN 2218-273X
Peer reviewed article  

Available in  Authors 

Author keywords
    planarians; wound healing and tissue regeneration; stem cells; redox molecules; reactive oxygen species; antioxidants

Authors  Top 
  • Bijnens, K.
  • Jaenen, V.
  • Wouters, A.
  • Leynen, N.
  • Pirotte, N.
  • Artois, T., more
  • Smeets, K.

Abstract
    A strict coordination between pro- and antioxidative molecules is needed for normal animal physiology, although their exact function and dynamics during regeneration and development remains largely unknown. Via in vivo imaging, we were able to locate and discriminate between reactive oxygen species (ROS) in real-time during different physiological stages of the highly regenerative planarian Schmidtea mediterranea. All ROS signals were strong enough to overcome the detected autofluorescence. Combined with an in situ characterisation and quantification of the transcription of several antioxidant genes, our data showed that the planarian gut and epidermis have a well-equipped redox system. Pharmacological inhibition or RNA interference of either side of the redox balance resulted in alterations in the regeneration process, characterised by decreased blastema sizes and delayed neurodevelopment, thereby affecting tails more than heads. Focusing on glutathione, a central component in the redox balance, we found that it is highly present in planarians and that a significant reduction in glutathione content led to regenerative failure with tissue lesions, characterised by underlying stem cell alterations. This exploratory study indicates that ROS and antioxidants are tightly intertwined and should be studied as a whole to fully comprehend the function of the redox balance in animal physiology.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors 


If any information here appears to be incorrect, please contact us
Back to Register of Resources
 
Quick links

MarBEF WIKI

Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC)
Outreach

Science
Responsive Mode Programme (RMP) - Marie Nordstrom, copyright Aspden Rebecca

WoRMS
part of WoRMS logo

ERMS 2.0
Epinephelus marginatus Picture: JG Harmelin

EurOBIS

Geographic System

Datasets

 


Web site hosted and maintained by Flanders Marine Institute (VLIZ) - Contact data-at-marbef.org