Experimentele bepaling van de weefselspecifieke turnoversnelheid van stabiele C en N isotopen bij de mariene grondel (Pomatoschistus minutus)
Van Den Driessche, P. (2005). Experimentele bepaling van de weefselspecifieke turnoversnelheid van stabiele C en N isotopen bij de mariene grondel (Pomatoschistus minutus). MSc Thesis. Katholieke Universiteit Leuven, Laboratorium voor Aquatische Ecologie: Leuven. 94 pp.
Related to:
Van Den Driessche, P. (2006). Experimentele bepaling van de weefselspecifieke turnoversnelheid van stabiele C en N isotopen bij de mariene grondel (Pomatoschistus minutus), in: Mees, J. et al. (Ed.) VLIZ Young Scientists' Day, Brugge, Belgium 31 March 2006: book of abstracts. VLIZ Special Publication, 30: pp. 87-92, more
In order to study the migration patterns of the marine sand goby Pomatoschistus minutus between the North Sea and the Schelde estuary using stable C and N isotopes, it is essential that the tissue specific turn over rates are known. Also, it is a prerequisite for this technique that an isotopic gradient between the two migration endpoints is present. The two main aims of this thesis were to assess the turn over rates for muscle, liver and heart tissue of P. minutus and to verify the C and N isotopic gradients in the Schelde estuary.To characterize the tissue specific turnover rates of the juvenile sand goby, we conducted an experiment in laboratory conditions for 90 days. During this experiment sand gobies were fed an isotopically different diet and were sacrificed after 10, 20, 30, 45, 60, 75 and 90 days. This way the change in d13C and d15N of muscle, liver and the heart tissue could be monitored and plotted against time and increased biomass. Based on the half life times (days) the three tissues could be ranked as follows, for d13C: (days): heart (6,2) < liver (10,65) < muscle (24,8). For d15N another ranking was found: liver (2,48) < muscle (23,79) < heart (24,2). In conformity with our expectations, a considerable influence of metabolic activity was demonstrated on the rate of isotopic change. Since the models based on increased biomass described more accurately the changes in d13C and d15N, compared to the time models, we recommend using these models when investigating the migration dynamics of P. minutus. Especially, the models describing the change in d13C and d15N of muscle, d13C of liver and d15N of heart were able to make accurate estimates of the experiment time. It was also our intention to investigate the effects of fasting on the d13C and d15N of the three tissues. When food was deprived for 20 days, a significant enrichment was only found for d15N in liver and for d13C in heart tissue. The experiment also provided some insight in the fractionation factors of the studied tissues. They seemed to be very dependent on the served diet and the specific tissue. Because of a technical failure it was impossible to establish the d13C and d15N gradients between the North Sea and the Schelde estuary.
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